Isolation of polymorphic microsatellite markers from the malaria vector Anopheles marajoara (Diptera: Culicidae)
نویسندگان
چکیده
Microsatellite-containing regions were isolated and characterized in Anopheles ( Nyssorhynchus ) marajoara , a primary vector of malaria parasites in northeastern Amazonia, Brazil. An enrichment protocol yielded 500 positive clones. We designed primers to amplify 40 unique microsatellites, 11 of which amplified cleanly and were polymorphic. A survey of 323 individuals showed that these loci are highly variable with the number of alleles ranging from 11 to 52, and expected heterozygosity ranging from 0.64 to 0.95. These markers will be useful for studies of population structure and intraspecific variation in A. marajoara .
منابع مشابه
Notes on the holotype of Anopheles marajoara Galvão & Damasceno (Diptera, Culicidae)
Anopheles subgenus Nyssorhynchus is a monophyletic Neotropical group (Sallum et al. 2000, 2002) that currently contains 32 named species (Harbach 2004). The most important vectors of human malaria parasites in Central and South America are members of the subgenus and include An. darlingi Root, 1926, An. albimanus Wiedemann, 1820 and An. marajoara Galvão & Damasceno, 1942 (Conn et al. 2002). Ano...
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